Remediation of heavy metals by reducing their mobility and bioavailability without removing them from soils is considered to be a cost-effective and an efficient method to address their toxicity for living organisms and soil health. The main objectives of the current study, therefore, were to investigate the potential of sugarcane bagasse biochar in reducing the bioavailability of soil cadmium (Cd) and chromium (Cr) and assessing the impact of biochar application on soil microbial activities and plant growth in metal-contaminated soil. Air-dried soil was artificially spiked with Cd and Cr metals by using Cd(NO3)(2) and Cr(NO3)(2) solutions. Biochar was homogenously mixed in metal-spiked soil. The experimental spiked soil was categorized as with and without biochar-amended soil. Mash bean crop was used as test plant. After crop harvesting, soil Cd and Cr were extracted by diethylenetriaminepentaacetic acid (DTPA) to estimate the mobility of heavy metals. Furthermore, the plant metal contents, microbial biomass carbon, urease activity, dehydrogenase (DHA) activity, total organic carbon, and plant biomass were determined. The Langmuir adsorption isotherms were studied for Cd and Cr in unamended soil, biochar-amended soil, and biochar only. The electronegative charges of biochar and biochar-amended soil were also measured using the zeta potential. The results show that the application of biochar to Cr- and Cd-contaminated soil significantly reduced their availability by 85 and 63\%, respectively. The application of biochar at 15 g kg(-1) prominently reduced the extractable Cd contents by 29 and 32\% in Cd-contaminated and Cr-Cd-contaminated soils, respectively. The Cr concentration in the plant decreased by 34 and 41\% in Cr-contaminated and Cr-Cd-contaminated soil compared with spiked soil without biochar. The Langmuir model achieved the best fit; its isotherm predicted the biochar's maximum adsorption capacity for Cd (0.42 mg g(-1)) and Cr (0.35 mg g(-1)). Biochar incorporation in Cd-Cr-contaminated soil significantly increased the microbial activity and mash bean biomass. Our findings indicate that the addition of sugarcane bagasse-derived biochar significantly reduces the Cd and Cr availability in single and co-contaminated soil. In addition, an increase in the microbial activity and plant growth and a significant reduction in the Cd and Cr uptake by mash bean are possible after biochar addition.