Purpose: The objective of this study was to measure the environmental impact of five different laboratory-scale constructed wetland (CW) treatment systems with varying design approaches, which have been employed to treat different types of wastewater. Moreover, the present study also assessed the feasibility of treating landfill leachate using four different hybrid wetlands built outdoors, and analyzed the environmental viability based on the life cycle assessment (LCA). Primarily, the choice of media materials has been the focus of evaluating the sustainability of the systems, as for each system the media materials cover major material consumption and define treatment performance. Methods: This study applied a life cycle assessment using the SimaPro software tool to quantify the environmental impacts from the constructed wetland systems. Primarily, the LCA has been applied by adopting the ReCiPe 2016 method with cross-validation using the Impact 2002+ method. Moreover, an uncertainty analysis has been performed to determine any uncertainties involved in the datasets, along with sensitivity analysis on the inventory. Results and discussions: As the results suggest, the systems employed for wastewater treatment using cement mortar have the highest environmental burden. In contrast, the natural media choices, sugarcane bagasse and coco-peat, have proved to be environmentally favorable. Media employment from recycled materials like brick and steel slag could significantly redeem the previous environmental burdens of these materials, providing treatment efficiency. However, the systems employed for landfill leachate treatment revealed the CW using brick chips as the most vulnerable system with regards to environmental concerns, implying that the media brick chips are certainly the major contributor behind this high leap in the scale. However, both the systems worked very well in the carcinogenic category, providing good treatment performance, and eventually exerting lesser impact. Conclusion: The overall assessments suggested choice of media materials are essential to deciding the sustainability of a CW design. However, the CW is more beneficial and environmentally friendly than the other treatment methods, until the design scale has a high capacity. Nevertheless, the choice of the LCA method is also significant, while measuring impact scales.