Vinasse is a high pollutant liquid residue from bioethanol production. Due to its toxicity, most vinasse is used not disposed of in water bodies but employed for the fertigation of sugarcane crops, potentially leading to soil salinization or heavy metal deposition. The anaerobic digestion of vinasse for energy production is the main alternative to fertigation, but the process cannot eliminate colored compounds such as melanoidins, caramels, or phenolic compounds. The treatment of raw vinasse with white-rot fungi could remove colored and persistent toxic compounds, but is generally considered cost-ineffective. We report the treatment of vinasse by an autochthonous Trametes sp. strain immobilized in polyurethane foam and the concomitant production of high titers of laccase, a high value-added product that could improve the viability of the process. The reuse of the immobilized biomass and the discoloration of raw vinasse, the concentration of phenolic compounds, BOD and COD, and the phytotoxicity of the treated vinasse were measured to assess the viability of the process and the potential use of treated vinasse in fertigation or as a complementary treatment to anaerobic digestion. Under optimal conditions (vinasse 0.25X, 30 degrees C, 21 days incubation, 2\% glucose added in the implantation stage), immobilized Trametes sp. causes a decrease of 75\% in vinasse color and total phenolic compounds, reaching 1082 U L-1 of laccase. The fungi could be used to treat 0.50X vinasse (BOD 44,400 mg O-2 L-1), causing a 26\% decolorization and a 30\% removal of phenolic compounds after 21 days of treatment with maximum laccase titers of 112 U L-1, while reducing COD and BOD from 103,290 to 42,500 mg O-2 L-1 (59\%) and from 44,440 to 21,230 mg O-2 L-1 (52\%), respectively. The re-utilization of immobilized biomass to treat 0.50X vinasse proved to be successful, leading to the production of 361 U L-1 of laccase with 77\% decolorization, 61\% degradation of phenolic compounds, and the reduction of COD and BOD by 75\% and 80\%, respectively. Trametes sp. also reduced vinasse phytotoxicity to Lactuca sativa seedlings. The obtained results show that the aerobic treatment of vinasse by immobilized Trametes sp. is an interesting technology that could be employed as a sole treatment for the bioremediation of vinasse, with the concomitant the production of laccase. Alternatively, the methodology could be used in combination with anaerobic digestion to achieve greater decolorization and reduction of phenolic compounds, melanoidins, and organic load. {[